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Abstract
The kinetics and regulatory mechanisms of T cell migration through the endothelium have not been fully defined. In experimental, filter-based assays in vitro, transmigration of lymphocytes takes hours, compared with minutes, in vivo. We cultured endothelial cell (EC) monolayers on filters, solid substrates, or collagen gels and treated them with TNF-α, IFN-γ, or both prior to analysis of lymphocyte migration in the presence or absence of flow. PBL, CD4+ cells, or CD8+ cells took many hours to migrate through EC-filter constructs for all cytokine treatments. However, direct microscopic observations of EC filters, which had been mounted in a flow chamber, showed that PBL crossed the endothelial monolayer in minutes and were highly motile in the subendothelial space. Migration through EC was also observed on clear plastic, with or without flow. After a brief settling without flow, PBL and isolated CD3+ or CD4+ cells crossed EC in minutes, but the numbers of migrated cells varied little with time. Close observation revealed that lymphocytes migrated back and forth continuously across endothelium. Under flow, migration kinetics and the proportions migrating back and forth were altered little. On collagen gels, PBL again crossed EC in minutes and migrated back and forth bu
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Orchestral Manoeuvres in the Dark
English band
This article is about the band. For their debut album, see Orchestral Manoeuvres in the Dark (album).
Orchestral Manoeuvres in the Dark (OMD) are an English electronic band formed in Meols, Merseyside in by Andy McCluskey (vocals, bass guitar) and Paul Humphreys (keyboards, vocals). Regarded as pioneers of electronic music, OMD combined an experimental, minimalist ethos with pop sensibilities, becoming key figures in the emergence of synth-pop; McCluskey and Humphreys also introduced the "synth duo" format to British popular music. In the United States, the band were an early presence in the MTV-driven Second British Invasion.
McCluskey and Humphreys led the Id, a precursor group, from to and re-recorded their track "Electricity" as OMD's debut single in Weathering an "uncool" image and a degree of hostility from music critics, the band achieved popularity throughout Europe with the anti-war song "Enola Gay", and gained further recognition via Architecture & Morality () and its three hit singles. Although later reappraised, Dazzle Ships () was seen as overly experimental, and eroded European support. OMD embraced a more radio-friendly sound on Junk Culture (); this change in direction led to greater success in